die folgenden Arrays stehen zur Verfügung:
( IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, GM-CSF, IFN-gamma, TNF-alpha)
Bestellnr: QAH-TH-1-1 / QAH-TH-1-2
( IL-1a, IL-1ß, IL-4, IL-6, IL-8, IL-10, IL-13, MCP-1, IFN-gamma,
TNF-alpha)
Bestellnr: QAH-INF-1-1 / QAH-INF-1-2
| RayBio®
Human Cytokine QuantibodyTM
Arrays
-Measure the concentration of 10 cytokines
in a single experiment
|
|
Raybio® Human Cytokine
Quantibody™ TH1/TH2 Array 1
( IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, GM-CSF, IFN-gamma,
TNF-alpha) |
|
Raybio® Human Cytokine
Quantibody™ Inflammation Array 1
( IL-1a, IL-1ß, IL-4, IL-6, IL-8, IL-10, IL-13, MCP-1, IFN-gamma,
TNF-alpha) |
|
|
In addition to
its matchless high-density cytokine antibody arrays, Raybiotech
Inc. is now proudly offering the Quantibody™ products for
simultaneous quantitative measurement of multiple cytokines.
The kit can be used for diverse sample types: serum, plasma,
cell lysate, conditioned media and other body fluid.
Our human cytokine Quantibody ™ Arrays use fluorescent detection
method, which increase the signal reproducibility and accuracy
than chemiluminescent and colorimetric platforms. After blocking
arrays with blocking buffer, the arrays are incubated with
samples. Unspecific bound proteins are then removed and the
arrays are incubated with a cocktail of biotin-antibodies
and then streptavidin-conjugated Alexa Fluor 555. The signal
can be detected with most of the microarray laser scanners.
Sixteen identical antibody
arrays are spotted on a piece of standard glass slide using
a non-contact arrayer. Each of the 10-cytokine specific capture
antibody is arrayed in quadruplicate, together with positive
and negative controls. Cytokine standards, whose concentration
has been predetermined and calibrated with international reference
standards, were provided to generate a five-point standard
curve of the 10 cytokines. In a real experiment, standard
cytokines and samples will be assayed in each array simultaneously
through a sandwich ELISA procedure. By comparing signals from
unknown samples to the standard curve, the unknown cytokine
concentration in the samples will be determined. |
The product comes with
a 16-well removable gasket to form a modular device. You can measure
10 different cytokines in quadruplicate from up to 16 samples per
slide within 4 hours. Four modules can be nested into a tray, which
matches a standard microplate and allows for automated robotic high
throughput process of 64 arrays simultaneously. As little as 50-µl
samples per well is required to generate 48 times more data than
traditional ELISA, especially precious when sample supply is limited.
Also, the cost per sample data point is only a fraction of the cost
of traditional ELISA.
High affinity antibodies were used
in our product. Each antibody pair has been optimized for its highest
sensitivity, as low as 5 pg/ml.
We also provide custom testing services.
You can simply send us your samples and we will do all assays for
you.
 |
Format: Standard glass slide with 16
removable wells
Sample Volume: 50-100 ul
Detection Range: 5-3000 pg/ml Standard Curve Range:
5-800 pg/ml
Signal Duration: 4 hours Reproducibility:
<20% well-to-well CV
Detection Method: Fluorescence.
Alexa Fluor 555 (Cy3 wavelengths 555 nm excitation, 655 emission) |
| PRODUCT |
UNIT |
CAT
# |
| Raybio®
Human Cytokine Quantibody™ TH1/TH2 Array 1
(IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, GM-CSF,
IFN-alpha, TNF-gamma) |
1
slide |
QAH-TH-1-1
|
| 2
slide |
QAH-TH-1-2 |
| Software |
QAH-TH-1-SW |
| |
|
|
| Raybio®
Human Cytokine Quantibody™ Inflammation Array 1
(IL-1a, IL-1ß, IL-4, IL-6, IL-8, IL-10, IL-13, MCP-1,
IFN-alpha, TNF-gamma) |
1
slide |
QAH-INF-1-1 |
| 2
slide |
QAH-INF-1-2 |
| Software |
QAH-INF-1-SW |
|
|
