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Unsere Angebote für November 2007
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Fibrinogen (FBG) is a homodimer of molecular mass 340 kDa, made up of two sets of alpha, beta, gamma polypeptide chains, and synthesized in the parenchymal cell of the hepatocyte and in the megakaryocyte (1). FBG plays a major role in coagulation, and both elevated and decreased levels have clinical significance. Upon cleavage by thrombin in the initial stages of coagulation activation, FBG self-assembles to yield a fibrin clot matrix that subsequently is crosslinked by factor XIIIa to form an insoluble network. FBG also binds to the platelet glycoprotein IIbIIIa receptor so as to form bridges between platelets, thus facilitating aggregation (2). Elevated plasma FBG has been identified as an independent risk factor for coronary atherosclerosis and ischemic heart disease (3, 4). Individuals with congenital absence of FBG, termed afibrinogenemia, have prolonged bleeding times.
The Human Fibrinogen ELISA kit is designed for detection of human FBG in plasma. This assay employs a quantitative competitive sandwich enzyme immunoassay technique that measures FBG in less than 3 hours. A murine antibody specific for FBG has been pre-coated onto a 96-well microplate with removable strips. FBG in standards and samples is competed by a biotinylated FBG sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The Mouse Fibrinogen ELISA kit is designed for detection of mouse FBG in cell culture media, and plasma samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse FBG in 3.5 hours. A polyclonal antibody specific for mouse FBG has been pre-coated onto a 96-well microplate with removable strips. FBG in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for mouse FBG, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The Rat Fibrinogen ELISA kit is designed for detection of rat FBG in cell culture media, and plasma samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rat FBG in 3.5 hours. A polyclonal antibody specific for rat FBG has been pre-coated onto a 96-well microplate with removable strips. FBG in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for rat FBG, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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