The AssayMax Human Albumin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human albumin in cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human albumin in less than 2.5 hours. A polyclonal antibody specific for human albumin has been pre-coated onto a 96-well microplate with removable strips. Albumin in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for human albumin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. The minimum detectable dose of albumin is typically 500 pg/ml. Intra-assay and inter-assay coefficients of variation were 4.7% and 7.1% respectively. No significant cross-reactivity or interference was observed.