The AssayMax Angiotensin II ELISA kit employs a quantitative competitive sandwich enzyme immunoassay technique which measures Angiotensin II in less than 3 hours. A polyclonal antibody specific for Angiotensin II has been pre-coated onto a 96-well microplate with removable strips. Angiotensin II in standards and samples is competed by a biotinylated Angiotensin II sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.The minimum detectable dose of angiotensin II is typically 50 pg/ml. Intra-assay and inter-assay coefficients of variation were 5.5% and 7.8% respectively.