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Fibronectin human ELISA Kit
(96-well plate)
more info
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355€
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Fibronectin mouse ELISA Kit
(96-well plate)
more info
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355€
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Fibronectin rat ELISA Kit
(96-well plate)
more info
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355€
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Fibronectin (FN) is a major component of the extracellular matrix and blood plasma, and is a specific ligand for several integrin adhesion receptors. FN plays an important role not only in cell adhesion and wound healing, but also in embryogenesis and hematopoiesis. FN is over-expressed in cardiovascular disease states such as atherosclerosis and myocardial infarction. Reduced levels of FN have been reported in patients with Disseminated Intravascular Coagulation (DIC) and low concentrations appear to correlate with a poor prognosis.
The AssayMax Human Fibronectin ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative competitive sandwich enzyme immunoassay technique that measures human plasma, serum and cell culture supernatant FN in less than 3 hours. A murine antibody specific for FN has been pre coated onto a 96-well microplate with removable strips. FN in standards and samples is competed by a biotinylated FN sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. The minimum detectable dose of FN is typically 40 ng/ml. Intra-assay and inter-assay coefficients of variation were 5.2% and 7.2% respectively.
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