|
Myeloperoxidase ELISA Kit
|
|
|
Human Myeloperoxidase ELISA Kit
(96-well plate)
more info
|
345€
|
Myeloperoxidase (MPO) plays an important role in host defense systems. This 140 kDa protein, composed of two heavy chains of 53kDa and two light chains of 15 kDa, was first discovered in the 1960s. The release of MPO from the granules of neutrophils and monocytes in response to the activation of leukocytes allows the conversion of hydrogen peroxide and chloride ions into hypochlorous acid (HOCl), a strong oxidizing agent. Although MPO serves an important purpose in the defense system, various studies show that MPO plays a role in several inflammatory conditions. An elevated MPO level has been linked to coronary artery diseases. In atherosclerosis, the hardening of artery walls, high concentrations of MPO and its oxidation products can be found in areas of atheroma. MPO has also been linked to vulnerable plaque and endothelial dysfunction.
Nitric oxide (NO) signals blood vessels to relax and dilate, but MPO’s use of NO as a substrate can lead to atherosclerosis and endothelial dysfunction. MPO also affects cholesterol levels as well through the oxidation of LDL and HDL. Other studies show that an elevated MPO level in serum and plasma indicate a higher risk for cardiovascular events in patients hospitalized for chest pain. Patients with low CRP and TnT levels but a high MPO level are at greater cardiac risk than patients with low CRP, TnT and MPO levels. In the study by Baldus, results indicate that an elevated MPO level occurs before myocardial injury and the increased MPO level indicates unstable plaque formations, therefore, making MPO level a good predictor of potential negative cardiac events. The minimum detectable concentration of the MPO ELISA assay as measured by 2SD from the mean of a zero standard is estimated to be 0.25 ng/ml.
|
