Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62490-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate PADI4-Ab in samples. An antibody specific for PADI4-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPADI4-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI4-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI4-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Protein-arginine deiminase type-4 antibody microplate
Human Protein-arginine deiminase type-4 antibody standard
Human Protein-arginine deiminase type-4 antibody detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Protein-arginine deiminase type-4 antibody (PADI4-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human PADI4-Ab. No significant cross-reactivity or interference between Human PADI4-Ab and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
PADI4 is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.The deduced 663-amino acid protein, approximately 50% identical to rat PAD types I, II, and III and 73% identical with rat keratinocyte PAD IV, is most conserved in the C terminal two-thirds of the sequence. Functional analysis indicated that the recombinant protein is fully active against arginine-containing substrates. Western blot analysis showed that expression of a 67-kD protein is induced by incubation of HL60 cells with differentiation factors.
Gene ID
23569
Alternative
PADI4-Ab; antibody for PAD; PAD4; PADI5; PDI4; PDI5; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase; type V; protein-arginine deiminase
Accession
Q9UM07
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 17.954,99 €
Preis: 17.954,99 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen