Human Anti-CSFV IgG Antibody (CSFV-Ab-IgG) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Anti-CSFV IgG Antibody microplate
Human Anti-CSFV IgG Antibody standard
Human Anti-CSFV IgG Antibody detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Classical Swine Fever Virus (CSFV), Bovine Viral Diarrhoea Virus (BVDV), and Border Disease Virus (BDV) are the 3 members of the genus Pestivirus within the family Flaviviridae. CSFV causes serious losses in the pig industry since it is highly contagious and pathogenic, and can cause widespread deaths. Pigs infected with CSFV may shed a high amount of virus before showing clinical signs of the disease. If animals survive an acute or sub-acute infection they develop antibodies or become chronically infected, a situation where pigs excrete the virus intermittently or continuously until death. In pregnant sows, CSFV is able to cross the placenta and infect fetuses. It causes abortions, fetal mummification and stillborns; if the infection occurs mid gestation (ca.50-70 days of pregnancy), weak or persistently viremic piglets can be born. These persistently infected piglets are able to spread the virus, due to the absence of an immune response. Occasionally pigs may be infected with BVDV or BDV. These infections in pigs are usually mild and self-limiting, but it is important to identify them from the CSFV infection.