Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62535-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAA2 in samples. An antibody specific for PRKAA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 microplate
Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 standard
Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKAA2. No significant cross-reactivity or interference between Human PRKAA2 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
5'-AMP-activated protein kinase catalytic subunit alpha-2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Alternative
PRKAA2; AMPK; AMPK2; PRKAA; 5 -AMP-activated protein kinase; catalytic alpha-2 chain; AMP-activated protein kinase alpha 2 catalytic subunit; AMPK-alpha-2 chain
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 16.839,95 €
Preis: 16.839,95 €
lieferbar

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