Human Solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62540-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC2A2 in samples. An antibody specific for SLC2A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC2A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC2A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC2A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Solute carrier family 2, facilitated glucose transporter member 2 microplate
Human Solute carrier family 2, facilitated glucose transporter member 2 standard
Human Solute carrier family 2, facilitated glucose transporter member 2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC2A2. No significant cross-reactivity or interference between Human SLC2A2 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Glucose transporter 2 (GLUT2) is a transmembrane carrier protein that enables passive glucose movement across cell membranes. It is the principal transporter for transfer of glucose between liver and blood, and for renal glucose reabsorption. In humans, this protein is encoded by the SLC2A2 gene.It has been proposed by genetics researchers in neonatal and maternal-fetal medicine at Harvard University Medical School and Beth-Israel Deaconess Hospital Medical Center, that this creates a problem for drug treated diabetic pregnancies in which glucose levels in the woman are uncontrolled, exposing her fetus to the possibility of neural tube and cardiac defects in the early-developing brain, spine, and heart.
Gene ID
6514
Alternative
SLC2A2; GLUT2;
Accession
P11168
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 16.839,95 €
Preis: 16.839,95 €
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