Human E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62549-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIM68 in samples. An antibody specific for TRIM68 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRIM68 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM68 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM68 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human E3 ubiquitin-protein ligase TRIM68 microplate
Human E3 ubiquitin-protein ligase TRIM68 standard
Human E3 ubiquitin-protein ligase TRIM68 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRIM68. No significant cross-reactivity or interference between Human TRIM68 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TRIM68 contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells.The deduced 485-amino acid protein has a calculated molecular mass of 56 kD. It has a RING finger domain, followed by a B box and a coiled-coil region. The deduced protein contains an N-terminal cys-his cluster, followed by a bipartite nuclear localization signal, a coiled-coil leucine zipper-like domain, and a C-terminal RET finger protein (TRIM27)-like domain.
Gene ID
55128
Alternative
TRIM68; FLJ10369; GC109; MGC126176; RNF137; SS-56; SS56; Ro/SSA1 related protein; SSA protein SS-56; ring finger protein 137
Accession
Q6AZZ1
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 16.839,95 €
Preis: 16.839,95 €
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