Human Fragile histidine triad (FHIT) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62589-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Fragile histidine triad (FHIT) ELISA Kit employs a two-site sandwich ELISA to quantitate FHIT in samples. An antibody specific for FHIT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFHIT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FHIT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FHIT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Fragile histidine triad microplate
Human Fragile histidine triad standard
Human Fragile histidine triad detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Fragile histidine triad (FHIT) ELISA Kit has high sensitivity and excellent specificity for detection of Human FHIT. No significant cross-reactivity or interference between Human FHIT and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
a member of the histidine triad gene family, encodes a diadenosine 5', 5'''-P1, P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.The last 21 amino acids at the C terminus of UBC9 appear to be unimportant for its biologic activity, since a UBC9 mutant harboring a deletion of these amino acids could still restore normal growth of yeast containing a temperature-sensitive mutation in the homolog UBC9 gene. Mutational analysis indicated that UBC9 was associated with the C-terminal portion of FHIT.
Gene ID
2272
Alternative
FHIT; AP3Aase; FRA3B; AP3A hydrolase; bis(5 -adenosyl)-triphosphatase; diadenosine 5 ; 5 -P1; P3-triphosphate hydrolase; dinucleosidetriphosphatase; tumor suppressor protein
Accession
P49789
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 17.954,99 €
Preis: 17.954,99 €
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