Human Monocyte chemotactic protein 4 (MCP-4) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62593-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Monocyte chemotactic protein 4 (MCP-4) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL13 in samples. An antibody specific for CCL13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Monocyte chemotactic protein 4 microplate
Human Monocyte chemotactic protein 4 standard
Human Monocyte chemotactic protein 4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Monocyte chemotactic protein 4 (MCP-4) ELISA Kit has high sensitivity and excellent specificity for detection of Human CCL13. No significant cross-reactivity or interference between Human CCL13 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family.The chemokines are secreted proteins involved in immunoregulatory and inflammatory processes. Naruse et al. (1996) identified on chromosome 17q11.2 a cluster of chemokines from the CC family (characterized by a cys-cys sequence motif) and distinguished from a second chemokine family that features a cys-xxx-cys (CXC) motif . They mapped 1 gene of the CC family (called NCC1 by them) to the cluster using a panel of somatic cell hybrids with known deletions. NCC1 was originally characterized as an expressed sequence tag (EST). The deduced order of genes from a YAC contig assembled for the region is cen--NF1--MCP3, MCP1, NCC1, I-309--Y1741 breakpoint--RANTES--LD78-gamma, AT744.2 , LD78-beta --NCC3, NCC2, AT744.1 ), LD78-alpha--NCC4--RARA --tel.
Gene ID
6357
Alternative
CCL13; CKb10; MCP-4; MGC17134; NCC-1; NCC1; SCYA13; SCYL1; CK-beta-10; monocyte chemoattractant protein 4; monocyte chemotactic protein 4; new CC chemokine 1; small inducible cytokine A13; small inducible cytok
Accession
Q99616
Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 12.857,65 €
Preis: 12.857,65 €
lieferbar

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