Mouse Transmembrane protease serine 6 (TMPRSS6) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70149-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Transmembrane protease serine 6 (TMPRSS6) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS6 in samples. An antibody specific for TMPRSS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPRSS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Transmembrane protease serine 6 microplate
Mouse Transmembrane protease serine 6 standard
Mouse Transmembrane protease serine 6 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Transmembrane protease serine 6 (TMPRSS6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMPRSS6. No significant cross-reactivity or interference between Mouse TMPRSS6 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TMPRSS6 shares 35% sequence identity with human matriptase/ST14 and spinesin/TMPRSS5, and 84.3% identity with mouse Tmprss6. It contains structural features characteristic of type II serine proteinases, including a short hydrophobic domain, a type II transmembrane sequence, 2 CUB domains, 3 LDL receptor repeats, and a C-terminal catalytic domain with structural hallmarks of serine proteinases. A potential phosphorylation domain is present at the cytoplasmic tail. Sequence alignment of the catalytic domains of TMPRSS6 and other type II serine proteinase family members permitted the identification of several conserved domains. Northern blot analysis of multiple human tissues revealed expression of a 3.5-kb TMPRSS6 transcript exclusively in fetal and adult liver.
Gene ID
71753
Alternative
TMPRSS6; RP5-1170K4.5; IRIDA; membrane-bound mosaic serine proteinase matriptase-2; type transmembrane serine protease 6
Accession
Q9DBI0

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Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
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