Mouse Tropomodulin-4 (TMOD4) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70154-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Tropomodulin-4 (TMOD4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMOD4 in samples. An antibody specific for TMOD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMOD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMOD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMOD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Tropomodulin-4 microplate
Mouse Tropomodulin-4 standard
Mouse Tropomodulin-4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Tropomodulin-4 (TMOD4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMOD4. No significant cross-reactivity or interference between Mouse TMOD4 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TMOD4 encodes a muscle-specific member of the tropomodulin family of actin-regulatory proteins. The encoded protein caps the pointed end of actin filaments preventing both elongation and depolymerization. The capping activity of this protein is dependent on its association with tropomyosin. Alternatively spliced transcript variants encoding different isoforms have been described.
Human TMOD4 encodes a deduced 345-amino acid protein. Northern blot analysis on human tissues detected a prominent 1.3- to 1.5-kb transcript in skeletal muscle. Three faint transcripts were detected: a 6.8-kb transcript in heart and skeletal muscle, and 2.4 and 3.0 transcripts in skeletal muscle.
Gene ID
50874
Alternative
TMOD4; RP11-68I18.7; DKFZp779I0852; SK-TMOD; actin-capping protein
Accession
Q9JLH8

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt und Sicherheitsdatenblatt) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Fragen zum Produkt?
 
Schließen