Mouse Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70157-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit employs a two-site sandwich ELISA to quantitate TMLHE in samples. An antibody specific for TMLHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMLHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMLHE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMLHE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Trimethyllysine dioxygenase, mitochondrial microplate
Mouse Trimethyllysine dioxygenase, mitochondrial standard
Mouse Trimethyllysine dioxygenase, mitochondrial detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMLHE. No significant cross-reactivity or interference between Mouse TMLHE and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Epsilon-N-trimethyllysine hydroxylase (EC 1.14.11.8) catalyzes the conversion of epsilon-N-trimethyllysine to beta-hydroxy-N-epsilon-trimethyllysine in the first step of L-carnitine biosynthesis.
The deduced 421-amino acid human TMLHE and 405-amino acid rat Tmlhe proteins have calculated molecular masses of 49.5 kD and 47.5 kD, respectively, and share 92% amino acid identity. Subcellular fractionation localized rat Tmlhe to mitochondria. Rat Tmlhe was synthesized as a 47.5-kD precursor that is processed to an approximately 43-kD protein, most likely through cleavage of a mitochondrial targeting signal. Gel filtration analysis showed rat Tmlhe as an 87-kD band, suggesting that Tmlhe forms a homodimer.
Gene ID
192289
Alternative
TMLHE; BBOX2; FLJ10727; TMLH; XAP130; butyrobetaine (gamma); 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2; epsilon-trimethyllysine 2-oxoglutarate; epsilon-trimethyllysine hydroxylase
Accession
Q91ZE0

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Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
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