Mouse Meckelin (TMEM67) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70164-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Meckelin (TMEM67) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM67 in samples. An antibody specific for TMEM67 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM67 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM67 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM67 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Meckelin microplate
Mouse Meckelin standard
Mouse Meckelin detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Meckelin (TMEM67) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM67. No significant cross-reactivity or interference between Mouse TMEM67 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TMEM67 localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). The human TMEM67 gene encodes a 995-amino acid protein, which they authors called meckelin, with a calculated unglycosylated weight of 108 kD. Human and rat meckelin share 84% identity. Meckelin was predicted to contain a signal peptide, at least 2 cysteine-rich repeats, and a 490-residue extracellular region with 4 N-linked glycosylated sites, followed by 7 transmembrane domains and a 30-residue cytoplasmic tail.
Gene ID
329795
Alternative
TMEM67; JBTS6; MECKELIN; MGC26979; MKS3; TNEM67; meckelin
Accession
Q8BR76

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Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
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