Mouse Transmembrane protein 55A (TMEM55A) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70177-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Transmembrane protein 55A (TMEM55A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM55A in samples. An antibody specific for TMEM55A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM55A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM55A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM55A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Transmembrane protein 55A microplate
Mouse Transmembrane protein 55A standard
Mouse Transmembrane protein 55A detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Transmembrane protein 55A (TMEM55A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM55A. No significant cross-reactivity or interference between Mouse TMEM55A and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TMEM55A catalyzes the degradation of phosphatidylinositol 4, 5-bisphosphate (PtdIns-4, 5-P2) by removing the 4-phosphate.The deduced 300-amino acid protein contains a central Cx(5)R phosphatase catalytic motif and 2 transmembrane domains near its C terminus, a characteristic of lysosomal transmembrane proteins. RNA dot blot analysis detected expression in all tissues examined. Fluorescence-tagged TMEM55A localized with membrane markers of late endosomes or lysosomes.Ungewickell et al. (2005) assayed the phosphatase activity of TMEM55A synthesized in insect cells and found that it specifically catalyzed removal of the 4-phosphate from PtdIns-4, 5-P2. It showed no activity toward other phosphatidylinositol substrates or inositol phosphates tested.
Gene ID
72519
Alternative
TMEM55A; DKFZp762O076; PtdIns-4; 5-P(2) 4-phosphatase type
Accession
Q9CZX7

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Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
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