Mouse Transmembrane protein 53 (TMEM53) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70179-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Transmembrane protein 53 (TMEM53) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM53 in samples. An antibody specific for TMEM53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM53 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM53 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Transmembrane protein 53 microplate
Mouse Transmembrane protein 53 standard
Mouse Transmembrane protein 53 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Transmembrane protein 53 (TMEM53) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM53. No significant cross-reactivity or interference between Mouse TMEM53 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TMEM53 has no paralogs but is predicted to have many orthologs across eukaryotes. The secondary structure of TMEM53 is predicted to consist of alternating pairs of alpha helices and beta sheets.The function of TMEM53 is not fully understood. It contains a domain of unknown function, DUF829, which is approximately 240 amino acids long. This domain has not been found in proteins other than TMEM53 and its orthologs.
Based on human and mouse EST profiles and a human tissue GEO profile, TMEM53 appears to be expressed ubiquitously at low levels in both normal and cancerous tissues.Transmembrane protein 53 has no paralogs. It does, however, have orthologs extending throughout eukaryotes, from primates to amoeba.
Gene ID
68777
Alternative
TMEM53; RP4-678E16.2; FLJ22353; NET4; novel DUF829 domain-containing protein
Accession
Q9D0Z3

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Menge: 48T
Lieferbar: In stock
Listenpreis: 413,24 €
Preis: 413,24 €
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