Mouse TM2 domain-containing protein 1 (TM2D1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse TM2 domain-containing protein 1 microplate
Mouse TM2 domain-containing protein 1 standard
Mouse TM2 domain-containing protein 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

TM2D1 is a beta-amyloid peptide-binding protein. It contains a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily and known to be important in heterotrimeric G protein activation. Beta-amyloid peptide has been established to be a causative factor in neuron death and the consequent diminution of cognitive abilities observed in Alzheimer's disease. This protein may be a target of neurotoxic beta-amyloid peptide, and may mediate cellular vulnerability to beta-amyloid peptide toxicity through a G protein-regulated program of cell death.BBP bound the 42-amino acid form of human beta-amyloid peptide in vitro with high affinity and specificity. Expression of a signaling-deficient dominant-negative BBP mutant suppressed sensitivity of human Ntera-2 neurons to beta-amyloid-mediated toxicity.

TM2D1; BBP; beta-amyloid binding protein