Mouse Tight junction protein ZO-2 (TJP2) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Tight junction protein ZO-2 microplate
Mouse Tight junction protein ZO-2 standard
Mouse Tight junction protein ZO-2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

TJP2 encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutation in this gene have been identified in patients with hypercholanemia. Alternate splicing results in multiple transcript variants. This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain whereas isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. In brain and skeletal muscle, only isoform A1 is detectable. Isoform C1 is found in normal as well as in most neoplastic tissues while isoform A1 is present almost exclusively in normal tissue.

TJP2; RP11-16N10.1; MGC26306; X104; ZO2; Friedreich ataxia region gene X104 (tight junction protein ZO-2); tight junction protein 2; tight junction protein ZO 2; zonula occludens protein 2