Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE70225-48T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TINAGL1 in samples. An antibody specific for TINAGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTINAGL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TINAGL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TINAGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Tubulointerstitial nephritis antigen-like microplate
Mouse Tubulointerstitial nephritis antigen-like standard
Mouse Tubulointerstitial nephritis antigen-like detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TINAGL1. No significant cross-reactivity or interference between Mouse TINAGL1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Tubulointerstitial nephritis antigen-like 1 has been cloned from mouse adrenocortical cells and is known to be closely associated with zonal differentiation of adrenocortical cells. In cell culture systems, TINAGL1 is a matricellular protein that interacts with both structural matrix proteins and cell surface receptors. However, the physiological roles of TINAGL1 and regulation of its expression are still not clearly understood.
In the present study, the expression and localization of TINAGL1 in peri-implantation mouse embryos was examined. During preimplantation, the expression of both Tinagl1 mRNA and TINAGL1 protein was increased just prior to implantation. In blastocysts, TINAGL1 expression was localized to the trophectoderm.
Gene ID
94242
Alternative
TINAGL1; ARG1; LCN7; LIECG3; TINAGRP; P3ECSL; TINAG-like 1; androgen-regulated gene 1; glucocorticoid-inducible protein; lipocalin 7; oxidized-LDL responsive gene 2; tubulointerstitial nephritis antigen-related pr
Accession
Q99JR5
Menge: 48T
Lieferbar: In stock
Listenpreis: 344,37 €
Preis: 344,37 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen