p35/25

Hersteller Abclonal
Kategorie
Typ Antibody Polyclonal
Specific against Human
Applikationen WB, IHC
Protein Familie Neuroscience
Menge 100ug
Host Rabbit
ArtNr A0197
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Calculated MW
34kDa
Immunogen
A synthetic peptide of human p35/25
Synonyms
CDK5R1, CDK5P35, CDK5R, MGC33831, NCK5A, p23, p25, p35, p35nck5a
Purity
Affinity purification
Storage Buffer
Store at -20C or -80C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
Cyclin-dependent kinases (CDKs) are serine/threonine kinases that are activated by cyclins and govern eukaryotic cell cycle progression. While CDK5 shares high sequence homology with its family members, it is thought mainly to function in postmitotic neurons, regulating the cytoarchitecture of these cells. Analogous to cyclins, p35 and p39 associate with and activate CDK5 despite the lack of sequence homology. CDK5 is ubiquitously expressed, but high levels of kinase activity are detected primarily in the nervous system due to the narrow expression pattern of p35 and p39 in post-mitotic neurons. A large number of CDK5 substrates have been identified although no discrete substrates have been attributed as a function of p35 vs. p39. Amongst many, substrates of CDK5 include p35 and p39. p35 is rapidly degraded (T1/2 <20 min) by the ubiquitin-proteasome pathway (1). However, p35 stability increases as CDK5 kinase activity decreases, and this is likely a result of decreased phosphorylation of p35 at Thr138 by CDK5 (2). NGF activates Erk and EGR1, and induces p35 expression in PC12 cells (3). Proteolytic cleavage of p35 by calpain produces p25 upon neurotoxic insult, resulting in prolonged activation of CDK5 by p25. Accumulation of p25 is found in neurodegenerative diseases such as Alzheimer's disease and Amyotrophic Lateral Sclerosis (ALS) (4-5).
Illustration
Western blot analysis of A2058 cell lysate using p35/25 antibody.
Gene Symbol
CDK5R1
GeneID (Human)
8851
GeneID
8851
Protein MW
307
SWISS
Q15078
Route
Synthetic Peptide

Arbeitsanleitung

A0197.pdf

Menge: 100ug
Lieferbar: In stock
Listenpreis: 289,30 €
Preis: 289,30 €
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