NF-kappaB1 p105

Hersteller Abclonal
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse, Rat
Applikationen WB
Protein Familie NF-KB Signaling
Menge 200ug
Host Rabbit
ArtNr A0037-200ug
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
*

DESCRIPTION

Calculated MW
105kDa
Immunogen
Fusion protein of humanNFKB1
Gene ID
4790
Swiss Prot
P19838
Synonyms
NFKB1; DKFZp686C01211; EBP-1; KBF1; MGC54151; NF-kappa-B; NFKB-p105; NFKB-p50; p105; p50
Isotype
IgG
Purity
Affinity purification
Storage Buffer
Store at -20? or -80?. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
Transcription factors of the nuclear factor ? B (NF-?B)/Rel family play a pivotal role in inflammatory and immune responses (1, 2). There are five family members in mammals: RelA, c-Rel, RelB, NF-?B1 (p105/p50), and NF-?B2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-?B is sequestered in the cytoplasm by I?B inhibitory proteins (3-5). NF-?B-activating agents can induce the phosphorylation of I?B proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-?B to enter the nucleus where it regulates gene expression (6-8). NIK and IKK? (IKK1) regulate the phosphorylation and processing of NF-?B2 (p100) to produce p52, which is then translocated to the nucleus (9-11). Following IKK-mediated phosphorylation of p105 NF-?B1 at multiple sites (Ser921, 923, 927, and 932) on its carboxy-terminus, SCF?-TrCP mediated processing produces the 50 kDa active form p50 (12, 13).
Recommended Dilutions
WB 1:500-1:1000
illustration
Western blot analysis of extracts of Jurkat cellline, using NFKB1 antibody.
Menge: 200ug
Lieferbar: In stock
Listenpreis: 444,40 €
Preis: 444,40 €
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