FasL

Hersteller Abclonal
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse, Rat
Applikationen WB
Protein Familie Lymphocyte Signaling
Menge 100ug
Host Rabbit
ArtNr A0234
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Calculated MW
31kDa
Immunogen
Recombinant proteinof human FasL
Synonyms
FASLG, APT1LG1, CD178, CD95L, FASL, TNFSF6
Purity
Affinity purification
Storage Buffer
Store at -20C or -80C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1, 2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3, 4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-alpha, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8, 9).
Illustration
Western blot analysis of extracts from various mouse tissues, using FASL antibody.
Gene Symbol
FasLG
GeneID (Human)
356
GeneID
356
Protein MW
281
SWISS
P48023
Route
Recombinant protein

Arbeitsanleitung

A0234.pdf

Menge: 100ug
Lieferbar: In stock
Listenpreis: 289,30 €
Preis: 289,30 €
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