Ku80

Hersteller Abclonal
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse, Rat
Applikationen WB, IHC
Protein Familie Lymphocyte Signaling
Menge 50ug
Host Rabbit
ArtNr A0884-50ug
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
*

DESCRIPTION

Calculated MW
83kDa
Immunogen
Recombinant protein of human XRCC5
Gene ID
7520
Swiss Prot
P13010
Synonyms
FLJ39089; KARP-1; KARP1; KU80; KUB2; Ku86; NFIV
Isotype
IgG
Purity
Affinity purification
Storage Buffer
Store at -20? or -80?. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.
Recommended Dilutions
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:20-1:50
illustration
Western blot analysis of extracts of HepG2 cellslines, using XRCC5 antibody.
Menge: 50ug
Lieferbar: In stock
lieferbar
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Arne Pelz

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