Antibodies

EHMT2

Hersteller Abclonal
Kategorie Produkte / Antibody
Typ Antibody Polyclonal
Specific against Human, Mouse, Rat
Isotype IgG
Applikationen WB, IHC
Menge 200ug
Host Rabbit
ArtNr A1247-200ug
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Background
G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1, 2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3, 4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12).
Gene Symbol
EHMT2
Gene Template
Human
GeneID
10919
Route
Recombinant Protein
SWISS
Q96KQ7
Protein MW (aa)
132kDa
Synonyms
EHMT2, BAT8, C6orf30, DKFZp686H08213, FLJ35547, G9A, KMT1C, NG36, NG36/G9a,
Purity
Affinity purification
Storage Buffer
Store at -20C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Illustration
Western blot analysis of extracts of Mouse spleen tissue, using EHMT2 antibody.
Menge: 200ug
Lieferbar: In stock
Listenpreis: 404,00 €
Preis: 404,00 €
lieferbar

Angebot anfordern

Technische Fragen zu diesem Produkt?


Salesmanager: Arne Pelz

Email: sales@hoelzel.de

Arbeitsanleitung

A1247-200ug.pdf