BrdU

Hersteller Abclonal
Kategorie
Typ Antibody Monoclonal
Specific against other
Applikationen FC, IF, IHC
Menge 200ug
Host Mouse
ArtNr A1482-200ug
Eclass 6.1 32160702
Eclass 9.0 42030590
Lieferbar
Immunogen
Bromodeoxyuridine
Synonyms
BrdU, Bromodeoxyuridine
Purity
Affinity purification
Storage Buffer
Store at -20C or -80C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The _Monoclonal Antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.
Gene Symbol
BrdU
Route
Synthetic Compound
Menge: 200ug
Lieferbar: In stock
Listenpreis: 289,30 €
Preis: 289,30 €
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