Goat anti-HCA67 / PSD3 Antibody

Goat anti-HCA67 / PSD3 Antibody
Principle Name: HCA67 / PSD3
Official Symbol: PSD3
All Name and Symbols: PSD3; EFA6R; ADP-ribosylation factor guanine nucleotide factor 6; HCA67; KIAA0942; hepatocellular carcinoma-associated antigen 67; pleckstrin and Sec7 domain containing 3; DKFZp761K1423
Accession Number(s): NP_056125.3; NP_996792.1
Peptide Sequence: DHRPETPSIKQKVT, is from C Terminus
Human Gene ID(s): 23362
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognize both reported isoforms (NP_056125.3 NP_996792.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Preliminary experiments in lysates of cell lines HEK293, HepG2, and K562 gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been
Reference(s): Wang Y, Han KJ, Pang XW, Vaughan HA, Qu W, Dong XY, Peng JR, Zhao HT, Rui JA, Leng XS, Cebon J, Burgess AW, Chen WF. Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies. J Immunol. 2002 Jul 15; 169(2):1102-9..PMID: 12097419