Description |
Goat anti-MEPI / SERPINI2 Antibody Principle Name: MEPI / SERPINI2 Official Symbol: SERPINI2 All Name and Symbols: SERPINI2; MEPI; PI14; PANCPIN; TSA2004; SERPIN12; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2; serpin peptidase inhibitor, clade I (pancpin), member 2; myoepithelium-derived serine protease inhibitor; protease inhibitor 14; Accession Number(s): NP_006208.1; NP_001012303.1 Peptide Sequence: KGDWKQKFRKEDTQ, is from internal region Human Gene ID(s): 5276 Non-Human GeneID(s): 67931 (mouse); 171149 (rat); Important Comments: This antibody is expected to recognise both reported isoforms (NP_006208.1; NP_001012303.1). Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 64000. Western Blot: Approx 40kDa band observed in Human Pancreas lysates (calculated MW of 46.1kDa according to NP_006208.1 and 47.4kDa according to NP_001012303.1). Recommended concentration: 0.3-1ug/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and of rare cells in the islets of Langerhans. Recommended concentration, 3ug/ml. Reference(s): Ozaki K, Nagata M, Suzuki M, Fujiwara T, Miyoshi Y, Ishikawa O, Ohigashi H, Imaoka S, Takahashi E, Nakamura Y. Isolation and characterization of a novel human pancreas-specific gene, pancpin, that is down-regulated in pancreatic cancer cells. Genes Chromosomes Cancer. 1998 Jul; 22(3):179-85. .PMID: 9624529 |