Goat anti-CBX3 / HP1 Gamma Antibody

Goat anti-CBX3 / HP1 Gamma Antibody
Principle Name: CBX3 / HP1 Gamma
Official Symbol: CBX3
All Name and Symbols: CBX3; HP1-GAMMA; chromobox homolog 3 (HP1 gamma homolog, Drosophila) ; HGNC:1553; HECH; HP1Hs-gamma ; HP1 gamma homolog; chromobox homolog 3; chromobox homolog 3 (Drosophila HP1 gamma); heterochromatin protein HP1 gamma; heterochromatin-like protein 1
Accession Number(s): NP_009207.2
Peptide Sequence: EAFLNSQKAGKEKD, is from internal region
Human Gene ID(s): 11335
Non-Human GeneID(s): 12417 (mouse); 297093 (rat);
Important Comments: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human, Mouse
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx. 20kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 ug/ml.
Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml.
Reference(s): Vakoc CR, Mandat SA, Olenchock BA, Blobel GA. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Mol Cell. 2005 Aug 5; 19(3):381-391. .PMID: 16061184