Goat anti-ZASP/ CYPHER / LDB3 Antibody

Goat anti-ZASP/ CYPHER / LDB3 Antibody
Principle Name: ZASP/ CYPHER / LDB3
Official Symbol: LDB3
All Name and Symbols: LDB3; ZASP; LIM domain binding 3 ; HGNC:15710; KIAA01613; KIAA0613; ORACLE; PDLIM6; PDZ and LIM domain 6; Z-band alternatively spliced PDZ-motif; CYPHER; FLJ35865; ldb3z1; ldb3z4
Accession Number(s): NP_009009.1; NP_001073583.1; NP_001073584.1; NP_001073585.1
Peptide Sequence: KSQNKPEDEADE, is from internal region
Human Gene ID(s): 11155
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Western Blot experiments gave bands at approx 90kDa and 35kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003
Reference(s): Selcen D, Engel AG. Mutations in ZASP define a novel form of muscular dystrophy in humans. Ann Neurol. 2005 Feb; 57(2):269-76. .PMID: 15668942