Goat anti-Duffy / FY / DARC Antibody

Goat anti-Duffy / FY / DARC Antibody
Principle Name: Duffy / FY / DARC
Official Symbol: ACKR1
All Name and Symbols: FY; DARC; HGNC:4035; Duffy blood group; CCBP1; GPD; CTA-134P22.3; CD234; FY; OTTHUMP00000060081; Duffy blood group, chemokine receptor; Duffy blood group antigen; Glycoprotein D; Dfy; GpFy; WBCQ1; Fy glycoprotein; OTTHUMP00000035293; OTTHUMP00000035294
Accession Number(s): NP_001116423.1; NP_002027.2
Peptide Sequence: HRAELSPSTENSSQLDFED, is from N Terminus
Human Gene ID(s): 2532
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, FC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3
Reference(s): Bruhl H, Vielhauer V, Weiss M, Mack M, Schlondorff D, Segerer S. Expression of DARC, CXCR3 and CCR5 in giant cell arteritis. Rheumatology (Oxford). 2005 Mar; 44(3):309-13. Epub 2004 Nov 30. .PMID: 15572394