Goat anti-NOTCH3 Antibody

Goat anti-NOTCH3 Antibody
Principle Name: NOTCH3
Official Symbol: NOTCH3
All Name and Symbols: NOTCH3; Notch homolog 3 (Drosophila); HGNC:7883; CADASIL; CASIL ; Notch (Drosophila) homolog 3; Notch homolog 3
Accession Number(s): NP_000426.2
Peptide Sequence: QLGPQPEVTPKRQ, is from C Terminus
Human Gene ID(s): 4854
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa
Immunohistochemistry: In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration, 4-6ug/ml.
Reference(s): Dang L, Fan X, Chaudhry A, Wang M, Gaiano N, Eberhart CG. Notch3 signaling initiates choroid plexus tumor formation. Oncogene. 2005 Sep 26; [Epub ahead of print] .PMID: 16186803