Goat anti-CCKAR Antibody

Goat anti-CCKAR Antibody
Principle Name: CCKAR
Official Symbol: CCKAR
All Name and Symbols: CCKAR; cholecystokinin A receptor ; HGNC:1570; CCK-A; CCK1-R; CCKRA; cholecystokinin type-A receptor; cholecystokinin-1 receptor
Accession Number(s): NP_000721.1
Peptide Sequence: FEASQKKSAKERKPST, is from internal region
Human Gene ID(s): 886
Non-Human GeneID(s): 12425 (mouse); 24889 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC, ICC, IF
Tested Species: Human, Rat
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Preliminary experiments in Human Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry: In paraffin embedded Human Pancreas, shows cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10ug/ml.
Reference(s): Beglinger C, Degen L, Matzinger D, D'Amato M, Drewe J. Loxiglumide, a CCK-A receptor antagonist, stimulates calorie intake and hunger feelings in humans. Am J Physiol Regul Integr Comp Physiol. 2001 Apr; 280(4):R1149-54. .PMID: 11247838