Goat anti-CYP3A4 Antibody

Goat anti-CYP3A4 Antibody
Principle Name: CYP3A4
Official Symbol: CYP3A4
All Name and Symbols: CYP3A4; cytochrome P450, family 3, subfamily A, polypeptide 4 ; HGNC:2637; CP33; CP34; CYP3A; CYP3A3; HLP; NF-25; P450C3; P450PCN1; P450-III, steroid inducible; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfami
Accession Number(s): NP_059488.2; NP_001189784.1
Peptide Sequence: KESRLEDTQKHR, is from internal region
Human Gene ID(s): 1576
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise both reported isoforms (NP_059488.2; NP_001189784.1) and it is not expected to cross-react with 3A5, 3A7 ot 3A43.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Preliminary experiments gave an approx 26-27kDa band in human liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57
Reference(s): Choi MH, Skipper PL, Wishnok JS, Tannenbaum SR. Characterization of testosterone 11{beta}-hydroxylation catalyzed by human-liver microsomal cytochromes P450. Drug Metab Dispos. 2005 Mar 11; [Epub ahead of print] .PMID: 15764715