Goat anti-TAK1 / MAP3K7 Antibody

Goat anti-TAK1 / MAP3K7 Antibody
Principle Name: TAK1 / MAP3K7
Official Symbol: MAP3K7
All Name and Symbols: MAP3K7 ; TAK1; mitogen-activated protein kinase kinase kinase 7 ; TGF1a ; TGF-beta activated kinase 1; transforming growth factor-beta-activated kinase 1
Accession Number(s): NP_003179.1; NP_663304.1
Peptide Sequence: AELDQDEKDQQNT, is from internal region
Human Gene ID(s): 6885
Non-Human GeneID(s): 26409 (mouse);
Important Comments: This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB Trf
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: In transfected HEK293 transiently expressing full-length Human MAP3K7 (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag
Reference(s): Omori E, Matsumoto K, Sanjo H, Sato S, Akira S, Smart RC, Ninomiya-Tsuji J. TAK1 is a master regulator of epidermal homeostasis involving skin inflammation and apoptosis. J Biol Chem. 2006 May 4; [Epub ahead of print] .PMID: 16675448