Goat anti-GPX4 (Isoform a and c) Antibody

Goat anti-GPX4 (Isoform a and c) Antibody
Principle Name: GPX4 (Isoform a and c)
Official Symbol: GPX4
All Name and Symbols: GPX4; glutathione peroxidase 4 (phospholipid hydroperoxidase) ; HGNC:4556; PHGPx; snGPx; glutathione peroxidase 4; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
Accession Number(s): NP_002076.2; NP_001034937.1
Peptide Sequence: EEPLVIEKDLPHY, is from C Terminus
Human Gene ID(s): 2879
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3ug/ml.
Reference(s): Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN. Mammalian selenoprotein thioredoxin/glutathione reductase: Roles in disulfide bond formation and sperm maturation. J Biol Chem. 2005 May 18; [Epub ahead of print] .PMID: 15901730