Goat anti-SIRT1 Antibody

Goat anti-SIRT1 Antibody
Principle Name: SIRT1
Official Symbol: SIRT1
All Name and Symbols: SIRT1 ; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ; RP11-57G10.3; SIR2L1 ; OTTHUMP00000060745; SIR2alpha; sir2-like 1; sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; sirtuin 1; sirtui
Accession Number(s): NP_036370.2
Peptide Sequence: EITEKPPRTQKE, is from internal region
Human Gene ID(s): 23411
Non-Human GeneID(s): 93759 (mouse); 309757 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Mouse
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 120kDa band observed in Mouse fetal Brain lysates (calculated MW of 80.3kDa according to Muose NP_062786.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Nat
Reference(s): Chen D, Steele AD, Lindquist S, Guarente L. Increase in activity during calorie restriction requires Sirt1. Science. 2005 Dec 9; 310(5754):1641. .PMID: 16339438