Goat anti-DAP10 / HCST Antibody

Goat anti-DAP10 / HCST Antibody
Principle Name: DAP10 / HCST
Official Symbol: HCST
All Name and Symbols: HCST; DAP10; hematopoietic cell signal transducer; KAP10; PIK3AP ; DNAX-activation protein 10; phosphoinositide-3-kinase adaptor protein
Accession Number(s): NP_055081.1; NP_001007470.1
Peptide Sequence: RSPAQEDGKVYIN, is from C Terminus
Human Gene ID(s): 10870
Non-Human GeneID(s): 23900 (mouse); 474146 (rat);
Important Comments: This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 40kDa band observed in human spleen lysates (calculated MW of 9.5kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30; 28
Reference(s): Upshaw JL, Arneson LN, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ. NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells. Nat Immunol. 2006 May; 7(5):524-32. Epub 2006 Apr 2. .PMID: 16582911