Goat anti-PRPF31 Antibody

Goat anti-PRPF31 Antibody
Principle Name: PRPF31
Official Symbol: PRPF31
All Name and Symbols: PRPF31; PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae); DKFZp566J153; NY-BR-99; PRP31; RP11 ; PRP31 pre-mRNA processing factor 31 homolog (yeast); pre-mRNA processing factor 31 homolog; pre-mRNA processing factor 31 homolog (yeast)
Accession Number(s): NP_056444.3
Peptide Sequence: KELGNSLDKCKNNEN, is from internal region
Human Gene ID(s): 26121
Non-Human GeneID(s): 68988 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 60kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 55.4kDa according to NP_056444.2). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 35kDa. Thi
Reference(s): Yuan L, Kawada M, Havlioglu N, Tang H, Wu JY. Mutations in PRPF31 inhibit pre-mRNA splicing of rhodopsin gene and cause apoptosis of retinal cells. J Neurosci. 2005 Jan 19; 25(3):748-57. .PMID: 15659613