Description |
Goat anti-Hap1 (mouse) Antibody Principle Name: Hap1 (mouse) Official Symbol: Hap1 All Name and Symbols: Hap1; huntingtin-associated protein 1; RP23-392I3.11, HAP-1, MGC31449 Accession Number(s): NP_034534.1 Peptide Sequence: QDAHSKRQQKQK, is from C Terminus Human Gene ID(s): Non-Human GeneID(s): 15114 (mouse); 29430 (rat); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Mouse, Rat Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Approx 100kDa band observed in Mouse and Rat lysates (calculated MW of 66.8kDa according to NP_034534.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.. Recommended co Reference(s): Rong J, McGuire JR, Fang ZH, Sheng G, Shin JY, Li SH, Li XJ. Regulation of intracellular trafficking of huntingtin-associated protein-1 is critical for TrkA protein levels and neurite outgrowth. J Neurosci. 2006 May 31; 26(22):6019-30. .PMID: 16738245 |