Goat anti-FABP2 Antibody

Goat anti-FABP2 Antibody
Principle Name: FABP2
Official Symbol: FABP2
All Name and Symbols: FABP2; fatty acid binding protein 2, intestinal ; FABPI; I-FABP; MGC133132 ; Fatty acid-binding protein, intestinal; intestinal fatty acid binding protein 2
Accession Number(s): NP_000125.2
Peptide Sequence: EGVEAKRIFKKD, is from C Terminus
Human Gene ID(s): 2169
Non-Human GeneID(s): 14079 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: A very strong approx 16kDa band observed in Human Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.2). In transfected HEK293 transiently expressing FABP2 a band of approx 18kDa is observed. This band is not observed in the
Immunohistochemistry: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration: 2-3ug/ml.
Reference(s): Takakura Y, Yoshioka K, Umekawa T, Kogure A, Toda H, Yoshikawa T, Yoshida T. Thr54 allele of the FABP2 gene affects resting metabolic rate and visceral obesity. Diabetes Res Clin Pract. 2005 Jan; 67(1):36-42. .PMID: 15620432