Goat anti-TIA1 Antibody

Goat anti-TIA1 Antibody
Principle Name: TIA1
Official Symbol: TIA1
All Name and Symbols: TIA1; TIA1 cytotoxic granule-associated RNA binding protein ; TIA1 cytotoxic granule-associated RNA-binding protein; TIA1 protein; cytotoxic granule-associated RNA-binding protein; p40-TIA-1 (containing p15-TIA-1)
Accession Number(s): NP_071320.1; NP_071505.1
Peptide Sequence: PKSTYESNTKQ, is from internal region
Human Gene ID(s): 7072
Non-Human GeneID(s): 21841 (mouse); 312510 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
An additional band at 80kDa and a very strong 26kDa is a
Immunohistochemistry: Paraffin embedded Human Thyroid. Recommended concentration: 3.75ug/ml.
Reference(s): Yang F, Peng Y, Murray EL, Otsuka Y, Kedersha N, Schoenberg DR. Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1. Mol Cell Biol. 2006 Dec; 26(23):8803-13. Epub 2006 Sep 18. .PMID: 16982678