Description |
Goat anti-FACT / SUPT16H Antibody Principle Name: FACT / SUPT16H Official Symbol: SUPT16H All Name and Symbols: SUPT16H; FACT; suppressor of Ty 16 homolog (S. cerevisiae); HGNC:11465; CDC68; FACTP140; FLJ10857; FLJ14010; SPT16/CDC68; chromatin-specific transcription elongation factor large subunit; FLJ34357; facilitates chromatin remodeling 140 kDa subunit Accession Number(s): NP_009123.1 Peptide Sequence: DLTNKEGKKPEEK, is from internal region Human Gene ID(s): 11198 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 8000. Western Blot: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band Reference(s): Belotserkovskaya R, Oh S, Bondarenko VA, Orphanides G, Studitsky VM, Reinberg D. FACT facilitates transcription-dependent nucleosome alteration. Science. 2003 Aug 22; 301(5636):1090-3. .PMID: 12934006 |