Goat anti-NCF4 / P40PHOX Antibody

Goat anti-NCF4 / P40PHOX Antibody
Principle Name: NCF4 / P40PHOX
Official Symbol: NCF4
All Name and Symbols: NCF4; P40PHOX; neutrophil cytosolic factor 4, 40kDa ; CTA-833B7.1; MGC3810; NCF; SH3PXD4; OTTHUMP00000028736; OTTHUMP00000043756; neutrophil NADPH oxidase factor 4; neutrophil cytosol factor 4; neutrophil cytosolic factor 4 (40kD)
Accession Number(s): NP_000622.2; NP_038202.1
Peptide Sequence: KDFPEEDDPTN, is from internal region
Human Gene ID(s): 4689
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. Th
Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4ug/ml.
Reference(s): Rioux JD, Xavier RJ, Taylor KD, Silverberg MS, Goyette P, Huett A, Green T, Kuballa P, Barmada MM, Datta LW, Shugart YY, Griffiths AM, Targan SR, Ippoliti AF, Bernard EJ, Mei L, Nicolae DL, Regueiro M, Schumm LP, Steinhart AH, Rotter JI, Duerr RH, Cho JH, Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. Nat Genet. 2007 May; 39(5):596-604. Epub 2007 Apr 15..PMID: 17435756