Goat anti-Neurexin 1 Antibody

Goat anti-Neurexin 1 Antibody
Principle Name: Neurexin 1
Official Symbol: NRXN1
All Name and Symbols: neurexin 1; DKFZp313P2036; NRXN1; FLJ35941; Hs.22998; KIAA0578; neurexin I
Accession Number(s): NP_004792.1 ; NP_620072.1
Peptide Sequence: KEKQPSSAKSSNKN, is from C Terminus
Human Gene ID(s): 9378
Non-Human GeneID(s): 18189 (mouse); 60391 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_004792.1 and NP_620072.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDD
Reference(s): Feng J, Schroer R, Yan J, Song W, Yang C, Bockholt A, Cook EH Jr, Skinner C, Schwartz CE, Sommer SS. High frequency of neurexin 1beta signal peptide structural variants in patients with autism. Neurosci Lett. 2006 Nov 27; 409(1):10-3. Epub 2006 Oct 10. .PMID: 17034946