GFP Expressing Human MDA-MB-435

Name of Products:GFP-Expressing Human Breast Cancer Cells (MDA-MB-435)
Catalogue Number:cAP-0049GFP
Product Format:Frozen Vial
Cell Number:> 5x10[5] cells/vial
General Information# Human MDA-MB-435 is a cell line isolated from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast. GFP-expressing MDA-MB-435 (GFP-MDA-MB-435) are selected from MDA-MB-435 cells after infected with lentiviruses expressing GFP with puromycin. The cells are shipped in a frozen vial with more than 5 x 105 cells/vial. Universal Full Growth Medium (cAP-01B) is recommended for cell culture and these cells have an average minimum population doubling levels > 20 when cultured following the detailed protocol described below).
Characterization of the Cells#
1.:MDA-MB-435 cells are tested negative for HIV-1, HBV, HCV, and mycoplasma.
Product Usage# The cells are offered for Research Use Only.
Shipping# Frozen Vials in a Dry Ice Package.
Handling of Arriving Cells# When you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage.
Protocols for Thawing the cells and subculture#
A):Pre-coating of T25 flasks- Add 2ml each Quick Coating Solution (cAP-01) into a T25 flask to cover the whole surface of the flask, 5 mins later, dispose the excessive coating solution by aspiration and the flask is ready to be used (although solution containing other extracellular matrix, i.e. gelatin, collagen, and fibronectin, can be used, make sure to optimize the conditions in advance).
B):Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask with 10ml of cAP-01B medium, cells usually become confluent with 5-7 days.
C):To passage the cells, rinse the cells in a T25 flask with 5ml HBSS (RT) twice; then add 2ml Trypsin/EDTA (RT) (cAP-23) into one T25 flask; gently dispose the excessive Trypsin/EDTA solution within 20 seconds by aspiration.
D):Leave the T25 flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins; or monitor the cells under a microscope until most of cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under microscope.
E):Add 5ml Trypsin Neutralization Buffer and spin down the cells with 800g centrifugation for 5 mins.
G):Re-suspend the cell pellet with 10 or 15ml Full medium and transfer 5 ml each into 2 or 3 pre-coated T25 flasks (for 1/2 to 1/3 subculture ratio).
H):Change medium every 2 or 3days and the cells usually become confluent within 7 days (when split at a 1/3 ratio).
Related Products#
Quick Coating Solution:cAP-01
Cardiac Myocytes Growth Medium:cAP-41
HBSS w/o Ca2+, Mg2+:cAP-11
Cell Freezing Solution (FBS):cAP-22
Cell Freezing Solution (Non-FBS):cAP-22B
Trypsin/EDTA Solution:cAP-23
Trypsin Neutralization Solution:cAP-28
ITS (100x):cAP-26
L-Glutamine-MAXIMUM (100x): cAP-27
Human Plasma Fibronectin Solution:cAP-42
Statement# Handling human tissue derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure.