Description |
Product Name: Macrophage-Colony Stimulating Factor Human Recombinant Synonyms: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF. Description: Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD.MCSF is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P09603 Amino Acid Sequence:MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution MCSF should be stored at 4C between 2-7 days and for future use below -18C.Please prevent freeze-thaw cycles. Formulation and Purity: The MCSF protein was lyophilized with 10mM sodium Phosphate, pH-8.0 & 50mM NaCl. Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found to be < 1.4-2.1ng/ml. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |