Fibroblast Growth Factor-basic Rat Recombinant

Product Name: Fibroblast Growth Factor-basic Rat Recombinant
Synonyms: HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b, Fibroblast Growth Factor-basic, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2.
Description: bFGF Rat Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.4 kDa.The bFGF2 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number: P13109
Amino Acid Sequence:MPALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.
Source: Escherichia Coli.
Physical Appearance and Stability: Lyophilized Rat bFGF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Rat FGF-2 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: FGF-b was lyophilized from 1mg/ml solution after extensive dialysis against 10mM Na2PO4, pH 7.5 and 50mM NaCl. Greater than 97.0% as determined by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50 range as determined by the dose-dependant proliferation of 3T3 cells was found to be less than 0.13-0.2ng/ml.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished form LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.